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When to use an inverted microscope?
Inverted microscope is useful for observing living cells or organisms at the bottom of a large container like a tissue culture flask under more natural conditions than on a glass slide, as is the case with a conventional microscope. On an inverted microscope this set-up is turned around giving the users more space plus the required proximity of the objective to the specimen.
Inverted microscopes have become useful for industrial applications, enabling us to look at more samples in a shorter period of time. Simply place the sample on the stage, focus onto the surface once and image it. Finished. The sample stays focused for all magnifications and further samples of the same sort are in focus alike.
With inverted microscopes, users look at samples from below since their optics are placed under the sample. This tool is used for life science research, because gravity makes samples sink to the bottom of a holder with aqueous solution and you don’t see a lot from above. But for some time, inverted microscopy has become increasingly popular for industrial applications as well. We have taken a look at which advantages inverted microscopy holds for industrial applications.
Advantages of Inverted Microscope
An inverted microscope works in the same direction the world does and gives the user greater freedom than an upright one. In terms of cost, it saves time and money in sample preparation.
- The Living cells can be observed by using an inverted microscope with a camera.
- Kohler Illumination can be used with Inverted Microscopy techniques for better magnification.
- Differential Interference Contrast of phase contrast microscopy can be used with an Inverted Microscope. This gives out the high contrasted images of biological samples.
- Phase-contrast optics can be used with the principle of an inverted microscope, to increase the image quality.
- Large and heavy biological specimens like metallurgical samples can be observed in the Inverted Microscopes. They are capable of observing the samples in minute detail.
- The microscopy technique allows researchers and scientists to attach a variety of digital recording equipment. Like a digital camera, it records the specimen‘s images with details.
- Inverted microscopy is famous for its maintenance of sterility of all biological samples. It maintains the sterility of all biological samples, it can be in its pure state after observations also.
- The inverted microscope is useful in the process of parasitology diagnosis.
Custom Configuration for Inverted Microscopes
Initially, when purchasing inverted microscopes, take into consideration the brand and the configuration you are required to have. Leading innovative brands are made available from Microscope International. You can choose from VanGuard, Optika, Euromex, Labomed, Motic, Unitron, Meiji Techno, to Accu-Scope. These brand names have an array of models such as: Inverted Biological Microscope, Inverted Fluorescence Microscopes, Digital Inverted Microscopes, Inverted Phase Microscope, Phase Contrast Inverted Microscope, Inverted Optical Microscopes, Labomed TCM400 Ergonomic Microscope, ACCU-SCOPE EXI-310-PH Inverted Microscope,
Inverted microscopes can be configured with: polarized, fluorescence, phase, and brightfield/darkfield. You can also choose among the models with ideal viewing head, objective types, and series. The online shop features search filters for you to conveniently find the specific instrument you are looking for. Customize your order by requesting a detailed quote from their team of experts.
“The inverted microscopy techniques are useful in magnifying images of biological samples. The instrument has basic components same as the normal microscopes but in reverse order. Due to its good feature of magnification, it has multiple applications in the biological world.” (SMACgig World).
Jacobs, A. (2014, November). How to use an Inverted Microscope. Retrieved February 2022, from PSU: https://sites.psu.edu/aaronmjacobs/wp-content/uploads/sites/20190/2014/11/Instruction-Set1.pdf
Greb, C. (2020, September 24). Factors to Consider When Selecting a Research Microscope. Retrieved February 2022, from Leica Microsystems: https://www.leica-microsystems.com/science-lab/factors-to-consider-when-selecting-a-research-microscope/
SMACgig World. Basic and Advantages of Inverted Microscopy. Retrieved February 2022, https://www.smacgigworld.com/blog/basics-and-advantages-inverted-microscopy.php
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